PTI, Photon Technology International, The Fluorescence Solutions Company

The Fluorescence Solutions Company




TM-50 Microscope based Fluorescence Lifetime Spectrofluorometer

  Buyer's Guide
Fluorescence Lifetime quote
TCSPC Lifetime Flyer

TimeMaster™ Lifetime Microscopy

PTI's TimeMaster™ Lifetime Microscopy system (TM-50) is a unique microscope-based fluorescence lifetime spectrometer. The TM-50 provides unsurpassed wavelength coverage from 240 nm (frequency-doubler option required) to 990 nm. A very intense pulses make it ideal for weak samples, while the low repetition rate prevents photodecomposition. The TM-50 uses PTI's specially designed photometer in conjunction with a proprietary fluorescence lifetime detection system. The TM-50 is a powerful, cost-effective, easy-to-use fluorescence lifetime system that can be used with virtually any fluorescence microscope.

Recommended applications include:

  • Fluorescence Lifetime Microscopy of Labeled Cells
  • Measurement of Intracellular Ions
  • Lifetime-based Oxygen Sensing
  • Lymphocytes Screening for Cancer
  • Multiphase Systems
  • Surface Photochemistry
  • Materials Research

Fluorescence detection techniques are well established in cell biology. Fluorescence microscopy is widely used to visualize microscopic structures and functional properties of cellular organelles. One of the most important features of fluorescence techniques is that commercially available fluorescent probes can provide information about various properties of their local environment in the cell. They can be used to monitor pH, viscosity, concentration of metal ions, polarity, molecular oxygen, conformational changes of the host environment etc. Measurable parameters that are affected by these factors are the fluorescence spectrum, lifetime, quantum yield and anisotropy of the fluorophores. While the steady-state (intensity) measurement can reflect some of these changes, the time-resolved (lifetime) measurement will give a more detailed insight into the mechanism of the process under study. Lifetime-based measurements are especially desirable, because unlike the intensity, the fluorescence lifetime is independent of the local fluorophore concentration. The Stray light is less of a problem in lifetime measurements and the technique can also be used to differentiate between fluorescent probes with overlapping spectra but different lifetimes. The time-resolved technique is also a powerful tool to study chemical systems such as colloids, liquid crystals, polymers, phase transition temperatures etc.

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