PTI, Photon Technology International, The Fluorescence Solutions Company

The Fluorescence Solutions Company




TM-50 Microscope based Fluorescence Lifetime Spectrofluorometer

Fluorescence Lifetime quote
TCSPC Lifetime Flyer


TimeMaster™ 50 comes with FelixGX software for instrumental control and includes new PowerFit-10 analytical software for fluorescence and phosphorescence lifetime analysis.

Through the new, powerful ASOC-10 USB interface FelixGX provides a full set of data acquisition protocols and controls the hardware for all system configurations and operating modes.

FelixGX Controls

  • Trigger and rep rate of pulsed laser
  • Timing of digital detector delay gate
  • Monochromator (optional)
  • Motorized slits (optional)

FelixGX Acquisition Modes

FelixGX also provides several acquisition modes for fluorescence decay measurements:

  • Fluorescence Decay with user controlled laser reprate, number of pulses averaged per point and user selectable linear, arithmetic or logarithmic timescale
  • Time-Resolved Emission scan (if optional emission monochromator is present) with user control of laser reprate, time delay of detector gate, number of pulses averaged per wavelength and scan step size  
  • Timebased scan with user control of laser reprate, time delay of detector gate, total time window and number of pulses averaged per point

PowerFit-10 Analytical Software

PowerFit-10 software (incorporated into FelixGX) provides powerful analytical package for decay data analysis. All modules include reconvolution algorithms, selection of data weighing (Poisson or analog), shift and offset parameters, statistical goodness-of-fit parameters (Chi-square, Durbin-Watson, Runs test, residuals and autocorrelation), as well as standard deviations of fit parameters.

PowerFit-10 Modules

  • Multi-exponential (1 to 4 lifetimes) decay
  • Multi-file multi-exponential (1 to 4 lifetimes)
  • Global (1 to 4 lifetimes) analysis
  • Anisotropy decay (free rotor, restricted rotor and other models)
  • Stretched exponential
  • Micelle quenching kinetics (Infelta-Groetzel-Tachiya model)
  • Exponential Series Method (ESM) unconstrained lifetime distribution analysis (up to 200 exp terms)
  • Maximum Entropy Method (MEM) lifetime distribution analysis (up to 200 exp terms)
  • Time-Resolved Spectra (TRES)
  • Decay Associated Spectra (DAS)


Fluorescence decay from a small area (5 x 5 micron) of fluorescein-labeled bead bracketed by photometer

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