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LaserStrobe™
Fluorescence Lifetime Spectrofluorometer

 
  System Enhancements
 
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Fluorescence Lifetimes

 

 

 

System Enhancements

 

Phosphorescence and Steady State (T1a)

PTI

Adds phosphorescence lifetime and steady state capability to systems, includes pulsed xenon source with QuadraScopic, computer controlled, monochromator for excitation, coupled to the second entrance port of sample compartment; added to the sample compartment second excitation channel optics; for detection a gated detector with 1527 PMT is coupled to the second exit port of the emission monochromator, one can select detectors by means of a flipping mirror.

The T-1A requires an open sample compartment port since it is directly coupled to the sample compartment and an open port on the current system's emission monochromator.

T1a Monochromator Specifications

Focal length 200 mm
Aperture ratio f/4 (calculated using grating width)
Wavelength range 180 nm to 24 microns
Reciprocal linear dispersion 4 nm/mm
Resolution 0.5 nm
Throughput 60% at 300 nm
Stray light 10 -5
Accuracy +/- 1 nm (using manual wavelength control)
Reproducibility +/- 1 nm
Optical path height 76 mm
Grating size 50 x 50 mm

 

T1a Detector Specifications

Detector Coming soon

 

T1a Lamp Specifications

Lamp type Pulsed xenon source
Wavelength range From 200 to 2,000 nanometers
Pulse repetition rate From 1 to 300 pulses per second
Pulse width Approximately 2 microseconds

 

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Steady State (T2)

PTI

For steady state measurement capability to lifetime systems, includes high intensity continuous xenon source with QuadraScopic, computer controlled, monochromator for excitation, coupled to the second entrance port of sample compartment; added to the sample compartment second excitation channel optics; for detection there is a high sensitivity photon counting/analog detector with 1527 PMT added to second exit port of emission monochromator, one can then choose between detectors by means of a flipping mirror.

The T-2 requires an open sample compartment port since it is directly coupled to the sample compartment and a open port on the current system's emission monochromator.

T2 Detector Specifications

Input +/- 15 VDC, 250 mA
High voltage -200 to -1,100 VDC manually adjustable (LCD display indicates actual cathode voltage)
External high voltage adjust 0 to +5 VDC (0 = -200 V, 5 = -1,100 V), continuously adjustable
Input regulation +/- 0.05% max. (for 15 V +/- 1 V input)
Load regulation +/- 0.05% max.
Ripple 100 mV p-p max.
Temperature coefficient +/- 0.0% max. (+5 to +40 deg. C)
Drift +/- 0.03%/hr max. (after 15 minute warm-up)

 

T2 Monochromator Specifications

Focal length 200 mm
Aperture ratio f/4 (calculated using grating width)
Wavelength range 180 nm to 24 microns
Reciprocal linear dispersion 4 nm/mm
Resolution 0.5 nm
Throughput 60% at 300 nm
Stray light 10 -5
Accuracy +/- 1 nm (using manual wavelength control)
Reproducibility +/- 1 nm
Optical path height 76 mm
Grating size 50 x 50 mm

 

T2 PMT Specifications

Spectral response 185 to 680 nm
Cathode sensitivity
      Luminous
     Radiant
60 µA/l
400 nm–60 mA/W
Anode sensitivity (at 1000 V)      Luminous
     Radiant
400 A/lm
400 nm–4.0 x 105 A/W
Low dark current 0.1 nA
Low dark counts (R1527P) - 10 cps

 

T2 Lamp Specifications

Lamp power capacity 75 to 200 watts
Heights 100 mm (3.9 inches)
Width 100 mm (3.9 inches)
Length 210 mm (8.3 inches)
Weight 1.9 kg (4.2 pounds)
Window diameter (D) 65 mm

 

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Extended Phosphorescence Detection (T3)

PTI

Extended phosphorescence detection option for fluorescence lifetime systems (for lifetimes beyond 500 nanoseconds), adds gated detector with 1527 PMT to second exit channel of emission monochromator, detector selection by means of flipping mirror.

T3 Detector Specifications

Detector Coming soon

 

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Dual Emission (T5)

PTI

Converts TM-3 lifetime system to L-format LaserStrobe™ system. Adds second emission monochromator, electrometer and 1527 PMT, sample compartment optics, filter holder and mechanical shutter.

T5 Emission Monochromator Specifications

Wavelength range 180 nm to 24 microns, continuously tunable (useful detection wavelength range dependent on grating and photomultiplier tube)
Resolution 0.5 nm
Accuracy +/- 0.5 nm
Reciprocal linear dispersion 4 nm/mm
Throughput 60% at 400 nm
Stray Light < 10 -5

 

T5 Stroboscopic Detector Specifications

Wavelength range 185 to 680 nm with standard R1527 PMT

 

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