PTI, Photon Technology International, The Fluorescence Solutions Company

The Fluorescence Solutions Company




Steady State Spectrofluorometer

  System Specifications
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System Specifications

PTI 460 Attomolar Sensitivity

The sensitivity of the QuantaMaster™ 400 UV VIS fluorescence spectrometer was assessed by testing fluorescein solution in 0.1 M NaOH.  Equipped with sensitivity enhanced optics, the fluorescein spectrum is clearly detectable in the attomolar range under the experimental conditions.


Detection Limit 400 attomolar fluorescein in 0.1 M NaOH
Signal to Noise Ratio 20,000:1 or better (350 nm excitation, 5 nm spectral bandpass, 1 s integration time)
Data Acquisition Rate 1,000,000 points/sec. to 1 point/1000 sec
4 analog (+/- 10 volts)
4 photon counting (TTL)
1 analog reference channel (+/- 10 volts)
2 analog (+/- 10 volts)
Emission Range 185 nm to 680 nm (optional to 1,010 nm)
Light Source High efficiency “ECO” friendly continuous Xenon arc lamp
Excitation Monochromator Coma-aberration corrected, asymmetrical, excitation optimized, Czerny-Turner design
Focal Length 300 mm
Emission Monochromator Coma-aberration corrected, asymmetrical, emission optimized, Czerny-Turner design
Excitation Grating 1200 line/mm 300 nm blaze
Emission Grating 1200 line/mm 400 nm blaze
Optional Grating 75 to 2400 line/mm and holographic models available
Wavelength Repeatability +/- 0.02 nm
Wavelength Resolution 0.022 nm
Detection Multimode: Photon Counting, 3 analog (fast, medium, slow response), direct and single shot transient digitizer (SSTD) mode
System Control Computer interface with spectroscopy software
Note: Specifications are based on standard system.


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