PTI, Photon Technology International, The Fluorescence Solutions Company

The Fluorescence Solutions Company




QuantaMaster™ 400
Steady State Spectrofluorometer

QM-400 Phosphorescence Fluorescence Spectrofluorometer Quote
Fluorescence Spectroscopy Brochure
Fluorescence Spectroscopy One Page Flyer


Whether you’re working in biology, medicine, physical or material science, protein measurements, DNA, FRET and others, the QuantaMaster™ 400 spectrofluorometer is the instrument for you! Samples are expensive but the sensitivity of the QuantaMaster™ 400 allows you use much less and measure more accurately and quickly than ever before. Myriad accessories and system enhancements makes the QuantaMaster™ 400 the perfect system for any application in fluorescence spectroscopy.

Below are some examples:


Protein Folding/Unfolding

Bovine Serum Albumin unfolding monitored with QuantaMaster 400 spectrofluorometer
Bovine serum albumin (BSA) unfolding monitored with PTI QuantaMaster™ 400 spectrofluorimeter equipped with the rapid temperature control accessory. Automated (macro) acquisition of BSA fluorescence spectra as a function of temperature showing the effect of thermal protein unfolding.


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Fluorescence Anisotropy

Fluorescence Anisotropy of BSA with QuantaMaster 400 Spectrofluorometer
Fluorescence anisotropy of bovine serum albumin (BSA) in PBS (pH=7.4) while ramping temperature with a QuantaMaster™ 400 equipped with dual emission channels and a rapid temperature change Peltier option.


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Fluorescence Resonance Energy Transfer (FRET)

3-D FRET titration
Titration monitored by FRET between Alexa-BSA complex and a Bodipy-labeled fatty acid.


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Bioluminescence and Chemiluminescence

Bioluminescence and Chemiluminescence emission
The unsurpassed sensitivity of the QuantaMaster™ 400 detection makes it a very capable instrument for measuring extremely weak chemiluminescence emission as illustrated by the cytochrome C/ hydrogen peroxide experiment.


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Semiconductor Research

Semiconductors Research with ZnO samples
Due to its dedicated accessories such as a well designed solid sample holder and excellent stray light rejection characteristics, the QuantaMaster™ 400 is an excellent choice for the semiconductors research. Here, clean spectra from strongly scattering ZnO samples were measured with the QuantaMaster™ 400 equipped with a double excitation monochromator.


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Electroluminescence and Photovoltaic Measurements

Electroluminesncen and Photovoltiac Measurements
The flexibility of the modular design makes it easy to utilize the QuantaMaster™ 400 for more specialized applications, such as electroluminescence or photovoltaic measurement. Here, the figure shows an electrical response of a photovoltaic cell illuminated with the QuantaMaster™ 400 excitation monochromator equipped with an NIR grating.


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QuantaMaster 400 fluorescence spectra of paper sample
Fluorescence spectra from a strongly scattering paper sample measured at several excitation wavelengths. System: QuantaMaster™ 400 with optional solid sample holder. The solid sample accessory allows precise alignment of the sample in the light beam with the use of rotation and translation adjustments (sample courtesy of Cameron Thomson, Institute of Paper Science and Technology, Georgia Institute of Technology).


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