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RatioMaster™ SL200
Fluorescence Microscopy

 
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FAQs

General FAQs

What kind of Microscopes can I use with the RatioMaster™?
The RatioMaster™ can be used with any standard Fluorescence microscope from Zeiss, Leica, Olympus,Nikon or Motic.

What ports can I use the RatioMaster™ on to illuminate my microscope?
PTI provides adaptors for both EPI and Direct port on the microscope. Two varieties are available. One for coupling to an existing EPI fluorescence light train, thus retrofitting the existing Mercury Lamp housing and a second type for Microscopes with NO EPI- fluorescence light train installed.

What ports can I use the RatioMaster's Photometer on?
For ease of use, any port that has a C mount can be used to mount the Photometer.

Does the RatioMaster™ come with its own power supply?
YES, the RatioMaster™ comes standard with all of the power supplies to be a stand-alone unit.

Is the RatioMaster™ easy to install?
YES, PTI supplies an easy to use manual. You can also have a PTI representative come to your lab to set up and train your on the RatioMaster™ system.

Light Transfer Portion (LLG) FAQs

How is the light delivered from the RatioMaster's excitation portion (DRX) to my microscope?
The light is collected from the DRX via a Liquid Light guide for maximum light delivery to the microscope.

Why would I use a Liquid light guide and not a fiber optic cable?
The DRX comes standard with a Liquid light guide due to the fact it delivers more than 30% more light than a fiber optic cable. A LLG is also more flexible than a fiber optic, where fibers can break if bent to far, a LLG is much more flexible with out compromising the throughput.

Can I use the RatioMaster's excitation portion to illuminate two microscopes?
Yes, you can have multiple microscope adaptors for the RatioMaster™ allowing the excitation portion of the system to be used for multiple microscopes in your lab.

How long is the Liquid Light Guide?
The LLG comes standard with a 2 meter length. However if you need to illuminate multiple scopes in your lab we do offer longer LLG's Check with your local representative for all of your options.

Photometer and PMT's FAQs

What wavelength region can I cover with the Standard RatioMaster™ SL200 Photometer?
The RatioMaster™ SL200 comes standard with a R1527 low noise PMT tube 185–680 nm.

Can I get a different PMT tube to get more QE in different wavelengths?
YES, PTI offers a variety of PMT tubes to match your application. Please contact us for all your options.

How do I focus the light into the Photometer?
It is as simple as focusing the image to your eyes on the microscope's Binoculars, by moving the focus of the objective you can focus the light coming out of the side port into the photometer.

What does the LCD read out on the PMT housing display?
The built-in LCD displays high voltage the PMT is set to or analog signal level.

Can I upgrade the single PMT photometer for dual emission in the future? (For two wavelengths simultaneous read out)
YES, PTI has an upgrade kit to allow the user to upgrade the UMP to dual emission. The kit includes all the necessary adaptors, PMT and PMT housing for the user to install.

What size filters can I use in the filter holder of the photometer?
The photometer uses a standard 1inch filter that can be changed by the user for multiple applications.

What does the eyepiece do for me?
The eyepiece is designed to allow the user to view the area of interest while moving the stage of the scope and using the UMP's aperture blades to narrow down onto the specific area you wish to measure.

How do I switch from the eyepiece to read out on the PMT?
It is as simple as flipping on a light switch, there is a thumb knob that you turn to direct the light from the eyepiece to the PMT.

What does the adjustable aperture blade do for me?
The adjustable aperture blades allow the user to create any size ROI on any area of the field of view.

What is the difference between Analog and Digital, Why do I need both?
Analog mode allows for high light levels with maximum dynamic range while with just a flip of a switch a user can go to Photon counting mode: for low signal, as in most ratio.

Can I use a Camera on the same port of the microscope as Photometer?
YES one key option that PTI has is a dual C mount adaptor that allows the user to install both Camera and Photometer onto the same port of the microscope.

RatioMaster™ SL200 Electronic and Software FAQs

What software can I use with the RatioMaster™?
The RatioMaster™ comes with the renowned FelixGX™ software for both process control and analytical capabilities of all of your data. I use Labview in my Lab.

Can I control the RatioMaster™ in Labview?
Yes the RatioMaster™ is fully compatible with labview. We have example Vi's to help you implement the system into Labview.

What Operating systems can I use with FelixGX™?
Currently, Windows XP, Windows 7, 32 and 64 bit.

I have a routine I need to run in the software can I write Macro's in FelixGX™?
Yes, the software comes with an easy to use VB based Macro recorder allowing for unsurpassed flexibility in acquisition, calculation and display of data.

Are there third party software packages that will run the RatioMaster™?
Yes, there are several software packages that will run the RatioMaster™. Check with your local representative for more information.

What kind of electronics do I need to run the RatioMaster™?
The RatioMaster™ comes with an electronic USB interface called the ASOC-10 to control both the excitation (DRX) and emission PMT's.

Do I need to install a Card in my PC like a PCI card?
No the ASOC-10 interface does not require you to install anything in your PC to control and read out the RatioMaster™ data.

Is it an advantage not to have a card in my PC?
Yes there are two main advantages. Ease of use, you can even run the system from a notebook. "speed critical data acquisition electronics" from the host PC, there is NO need for an interface card to be plugged into the host computer bus. The host computer runs a controlling program and communicates instructions to the ACOC-10. This process off loads the critical data acquisition tasks and frees up the host PC's processor. Since no data capture boards are on the host PC bus, hardware conflicts have essentially been eliminated, thus stabilizing the remote data capture system.

What kind of interface to my PC does the ASOC-10 have?
The standard interface is via USB 2.0 connection.

Can I run other instruments in my lab from the ASOC-10?
Yes, the ASOC-10 comes with (4) Analog, IN (2) TTL IN and (2) TTL OUT , (4) Digital Inputs as well as (4) DAC’s

Can I use the RatioMaster™ with a MAC?
No, at this time PTI does not offer support for Mac OS. However Intel based Macs can run Windows XP via Apple's "BootCamp". FelixGX™ can run on Windows XP, Win7 using Apple's "BootCamp".

Can I export my data from FelixGX™ into Excel?
Yes, PTI has an easy to use export function.

 

 
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