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EasyRatioPro
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EasyRatioPro

EasyRatioPro with Warp Drive! PTI gets back to it's grass roots, offering a completely new revolutionary user interface for the collection and analysis of ratio-metric imaging data for calcium, pH, and intracellular ion imaging!

EasyRatioPro is the most comprehensive system of its kind, EasyRatioPro offers Imaging researchers superb quality and efficiency through one intuitive, integrated production environment.

This high-resolution system embodies the latest PTI innovations, incorporating cutting-edge technology to deliver unprecedented image quality and price/performance. Featuring dramatic power, high speed streaming Image Acquisition, brand new high-resolution camera support and peripheral options, abundant channel wavelength count and I/O capacity, extensive flexibility and much more, EasyRatioPro gives you control over your Imaging world like never before.

As part of our work in muscle function, our study of the retention of function in HEK293 cells probed by cellular Ca2+ release in response to caffeine was greatly facilitated by the targeted functionality of our PTI EasyRatioPro instrumentation and software.

–Dr. Gerhard Meissner
University of North Carolina School of Medicine

For more details click here.

The most powerful imaging acquisition engine for measuring calcium, pH and intracellular ions

Dr. Derek Damron
Kent State

What's in the Box

  • DeltaRAM X™ monochromator
  • 2 meter liquid light guide
  • Labmaster PCI interface & I/O
  • EasyRatioPro Software
  • Warp Drive human user interface
  • Imaging workstation with 19" monitor
  • Installation and training
EasyRatioPro

General Applications

Calcium Measurement

  • Intracellular free Ca2+
  • Mitochondrial Ca2+
  • Endoplasmic reticulum (ER) Ca2+ pool
  • Lysosomal Ca2+
  • Extracellular near membrane Ca2+

Other Ions

  • Intracellular Mg2+
  • Intracellular Mn2+
  • Intracellular Zn2+
  • Intracellular Sr2+
  • Intracellular Na+
  • Intracellular Cl-
  • Cytosolic labile iron pool (LIP)
  • Iron homeostasis

Membrane Potential

  • Plasma membrane Potential
  • Mitochondrial Membrane Potential

pH

  • Intracellular pH
  • Vacuolar pH
  • Lysosomal pH

GFP & Variants

  • Identifying cells coexpressing GFP for analysis
  • Use mitoGFP to visualize mitochondria and measure mitochondrial calcium [Ca2+]m
  • Use ratiometric GFP (redox-GFP) to measure cellular/mitochondria reduce/oxidize reaction
  • HEK-293 cells transfected with the CaSR-pEGFP vector
  • ECFP
  • EYFP

FRET

  • Donor Excitation Spectroscopy
  • Emission intensity
  • Donor emission intensity
  • Acceptor emission intensity
  • Ratio of donor/acceptor emission intensity
  • Measure molecular proximity
  • Binding of enzyme to itssubstrate
  • Measure Endoplasmic reticulum (ER) Ca2+

Other Measurements

  • Calcium imaging of Single HEK 293 cell
  • Taste Bud
  • Pea Pollen tubes
  • Respiratory burst & reactive oxygen species (ROS)
  • NAD(P)H
  • Changes in CaM availability
  • Demonstration of selective plasma membrane permeabilization by digitonin in HEK293 cells
  • Detection by immunofluorescence of PfCRT expression in HEK293 cells
  • Receptor-mediated Glutamate release (by following NADH signal)
  • Fura-dextra
  • Cellular GSH level
  • FL-aldosterone efflux
  • Latency of response to exogenous ATP or ADP.
  • Membrane traffic
  • Liposome
  • ER membrane
  • Cell contractile activity & cell Size
  • Cell Volume Measurement
  • Phagocytosis
  • Cell injury or death by PI fluorescent
  • Metal induced cytotoxity

Calcium-related Measurement

  • Ca2+ Waves
  • Ca2+ wave quantitation
  • Ca2+ wave induced bymechanical stimulation
  • Ca2+ wave induced by mechanical wounding
  • Ca2+ wave propagation
  • In non-contacting cells
  • Over a physical gap
  • Ca2+ Oscillation induced by:
    • Hg2+
    • K+
    • Heat
    • ATP
    • Dopamine
    • FSH
    • Caffine
    • Ca2+ mobilization
    • Ca2+ homeostasis
    • Propagated cytoplasmic Ca2+cyt responses along the DVR wall.
  • Simultaneous Measurement of intracellular Ca2+ and:
    • Cell volume
    • Phygocytosis
    • Cl-
    • Oxidase activation
    • Ca2+ -CaM
  • And Many More!

Non Ratiometric

Application

Dye

Chloride

SPQ SPA
MQAE

 

 

 
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