PTI, Photon Technology International, The Fluorescence Solutions Company

The Fluorescence Solutions Company

 

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FluoDia™
Plate Reader

 
  Specifications
 
Fluorescence Plate Reader
 
QM-40 Plate Reader Quote
 
Fluorescence Plate Reader Brochure
 
 
 
Fluorescence Plate Reader
 

Specifications

General

External communication RS-232C or USB
Bar-code reader (exclusive for key interface). 
All bar-code up to "16" characters.
Computer requirements PC computer with a CD-ROM, a Windows NT 4.0, Windows 2000 Windows XP Windows 7 operation system, a serial port is required.
Power supply 120 V/230 V/100 V, 50/60 Hz
Power consumption Approx. 250 VA (~ 330 VA when heater plate is used)
Dimensions (w x d x l) 380 x 505 x 200 mm
Weight Approx. 20 kg
Environmental requirements
     Temperature 15–30°C
     Relative humidity 30–90% (no condensation)

 

Measurement

Measurement method Photon counting
Excitation light source 50 W quartz halogen lamp
Detector Side-on type PMT
Detector wavelength range 185–830 nm
Light guide Quartz fiber (1.4 mm) & condenser lens
Optics Top-to-top
Light axis for measurement Z-axis
Optics adjustment Optional Z-axis adjustment
Excitation wavelength range 320–830 nm
Excitation/emission wavelength Interference filter
Excitation/emission filter ports 4 each for ex/em
Excitation/emission switching Automatic (software controlled)
Stage Auto X - Y stage with automatic sensing of the well-center

 

Performance

Linearity Correlation coefficiency ≥ 0.995
Sensitivity 2.4 fmol/well fluorescein; 4-MUF: 0.1 pmol/well
Reproducibility CV £ 1.5%
Dark level ≤ 3 x 10-4% full scale
Stability ≤ 3 x 10-4% full scale/8 hours
Dynamic range 0 –16,777,215 counts
Gating time 10 msec–10 sec (can be setup for every 10 msec)
Measurement time
Approx. 51 sec/96 wells (if measured per well)
Dual wavelength Approx. 67 sec/96 wells (if measured per plate)
Single wavelength Approx. 40 sec/96 wells , Approx. 110 sec/384 wells
Number of averages 1–100 times/well
Fluorescence intensity calibration Automatic calibration using the exclusive calibration plate
Sample mixing Linear and butterfly mixing.  Time and velocity software controlled.
Temperature control
     Range Room temperature +5°C–75°C
     Heating stage Flat heater plate, 96-well or 384-well heater plate, dual P.I.D. control. 
     Thermal fuse Built-in (123°C)
     Incubation method Temperature stepping, simple incubation or incubation
     Accuracy ± 0.3°C
     Homogeneity ± 0.3°C
     Rise time ≤ 6 min. (up to 75°C from R.T.)
Temperature rise time of sample (rise time from 20°C to 68°C)
≤ 5 min. without pre-heating
≤ 3 min. if pre-heated at 45°C
≥ 1 min. if pre-heated at 68°C
Plate formats 6-384 well plates, strip tubes, 1536 well plates (optional)
Plate Dimensions
     Width 86.0 ± 0.8 mm
     Length 128.0 ± 0.8 mm
     Height 25 mm (without use of heater plate)   Approximately 20 mm with heater plate

 

 
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