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A Product for every Application!

PTI products provide a range of spectroscopy applications and since most of our systems have an Open Architecture design, you can find the right product to for all of your applications and you can adapt your spectrofluorometer to the demands of your research with our many accessories and system enhancements.

 

QuantaMaster™ Applications

PTI-QuantaMaster Steady State Spectrofluorometer

 

QuantaMaster™ 300

  • Protein folding
  • Phosphorescence Lifetimes
  • Fluorescence suppression
  • Lanthanide emission and lifetimes
  • Solid-state phosphorescence
  • Delayed fluorescence
  • Time resolved spectra
  • Gated emission spectra
  • Gated excitation spectra
  • Microseconds to seconds kinetics
  • DNA binding
  • Intracellular ion concentrations (Ca++, pH, etc...)
  • Spectra and lifetimes of phosphors and inorganic materials
  • Membrane potential
  • FRET
  • LRET
  • Stopped Flow
  • Chemi-and bioluminescence
  • More...

QuantaMaster™ 400

  • Millisecond kinetics
  • DNA binding
  • Intracellular ion concentrations (Ca++, pH, etc.)
  • Phosphorescence
  • Membrane potential
  • FRET
  • Polarization/Anisotropy
  • Stopped Flow
  • Chemi-and bioluminescence
  • Fluorescence kinetics
  • Fluorescence ratios
  • Excitation ratios
  • Dynamic anisotropy
  • Steady state
  • Fluorescence
  • Solid fluorescence
  • Temperature dependant fluorescence
  • Intracellular Ion Determination (Ca++, pH, etc…)
  • LRET
  • Intrinsic Fluorescence of Proteins
  • Photoluminescence of OLED devices
  • Thin Films
  • Single Photon Counting
  • Analog Detection
  • Quantum Yield studies of solids and solutions
  • 4 Position peltier for higher throughput when doing long protein folding studies.
  • Impurity detection
  • Cell suspension work
  • More...

QuantaMaster™ 50

  • Photochemistry
    Singlet oxygen is frequent byproduct
  • Geology
    NIR luminescence of minerals
  • Forensic science
    Identifying forged documents
  • Photobiology and photomedicine
    Singlet oxygen detection (1270 nm)
  • Cancer treatment
    Photodynamic therapy (PDT)
  • Photobiology
    Photodegradation caused by singlet oxygen
  • Photosensitized oxidations
    Photooxidation of environmental pollutants
  • Optical fiber communication
    Optical amplifiers (e.g. chelated Er++ , 1540 nm)
  • Agriculture
    Development of environmentally friendly pesticides
  • More...

QuantaMaster™ 80

  • Intracellular ion concentrations (Ca++, pH, etc.)
  • FRET
  • Membrane fluidity
  • Beta blockers
  • RNA and DNA
  • Membrane potential
  • Multiple probe experiments
  • Oxidants
  • Steady state fluorometry
  • More...

 

TimeMaster™ Applications

PTI--Lifetime

 

LaserStrobe™

  • Lifetimes of very weak or diluted fluorophores
  • Complex, heterogeneous kinetics
  • Lifetime and time-resolved spectra of tyrosine and tryptophan fluorescence in proteins (frequency-doubler option required)
  • Micellar studies
  • Polymers
  • Membranes
  • Porphyrins and chlorophylls
  • Excited state electron and proton transfer
  • Analytical and environmental assays
  • Phosphors, LEDs and solid state devices
  • Fluorescence resonance energy transfer (FRET)
  • Time-resolved fluorescence anisotropy
  • More...

 

RatioMaster™ Applications

PTI--RatioMaster Ionic Detection

 

RatioMaster™

Excitation-Shifted

Application

Dye

Calcium Fura-2,
Fura PE3
FFP18 Quin-2
Fura-Red
Sodium SBFI
BCECF
HPTS
SNAFL®
DM-NERF®
CI-NERF®
Potassium PBFI
Magnesium Mag-Fura-2
Mag-Fura-5
Membrane Potential Di-4-ANEPPS
Di-8-ANEPPS

Emission-Shifted

Application

Dye

Calcium Indo-1
pH SNARF-1®
SNARF-2®
Magnesium Mag-Indo-1
Membrane Fluidity Pyrene Fas
PATMAN
Free Fatty Acids ADIFABTM
Membrane Potential JC-1
Lipids, phoholipids Nile Red
Nucleic acids Acridine
Orange

Calcium-related Measurement

  • Ca2+ transients
  • Ca2+ mobilization
  • Ca2+ homeostasis
  • Ca2+ waves
  • Ca2+ oscillation
  • Ca2+ spikes
  • Ca2+ entry
  • Capacitative Calcium Entry (CCE)

Calcium Measurement

  • Intracellular free Ca2+
  • Mitochondrial Ca2+
  • Endoplasmic reticulum (ER) Ca2+ pool
  • Lysosomal Ca2+
  • Extracellular near membrane Ca2+

Simultaneous Measurement of intracellular a2+ and:

  • Cell volume
  • Cell contraction: Edge detection device
  • Membrane current: Patch Clamps
  • Phygocytosis
  • Cl-
  • Oxidase activation
  • Ca2+-CaM

Membrane Potential

  • Plasma membrane Potential
  • Mitochondrial Membrane Potential

pH

  • Intracellular pH
  • Phagosomal pH
  • Vacuolar pH
  • Lysosomal pH
  • More..

Non-Ratiometric

Application

Dye

Calcium Fluo-3
Calcium Green™
Calcium Orange™
Calcium Crimson™
Rhod-2
Chloride SPQ SPA
MQAE
HIV protease HIV protein substrate
F-actin phallotoxins
PKC Fim-1
Rim-1

Dual Dye

Application

Dye

Nucleic acids DAPI and FITC
FITC and APC
Fura Red™ and BCECF
FITC and Texas Red®
DAPI and PE

FRET

  • Measure molecular proximity
  • Binding of enzyme to its substrate
  • Measure Endoplasmic reticulum (ER) Ca2+
  • Donor Excitation Spectroscopy
  • Emission intensity
  • Donor emission intensity
  • Acceptor emission intensity
  • Ratio of donor/acceptor emission intensity

Common FRET Pairs

  • EGFP / DSRed2 FRET
  • CFP / DsRed2 FRET
  • BFP / DsRed2 FRET
  • CoralHue M-Cyan / K-Orange
  • GFP / Rhod2
  • FITC / CY3 FRET
  • FITC / TRITC FRET
  • YFP / TRITC FRET
  • CY3 / CY5 FRET
  • CFP / YEP FRET
  • BFP / GFP FRET

Other Ions

  • Intracellular Mg2+
  • Intracellular Mn2+
  • Intracellular Zn2+
  • Intracellular Sr2+
  • Intracellular Na+
  • Intracellular Cl-
  • Intracellular Ba2+
  • Divalent cation influx
  • Labile iron pool (LIP)
  • Iron homeostasis
  • Na+ / H+ exchange
  • H+ / HCO3- exchange
  • Pf and PCO

Other Measurements

  • Respiratory burst & reactive oxygen species (ROS)
  • NAD(P)H
  • Nitric Oxide (NO)
  • Intracellular GSH
  • Identifying cells coexpressing GFP for analysis
  • Use ratiometric GFP (redox-GFP) to measure cellular/mitochondria reduce/oxidize reaction
  • Calcein-based cell viability assay
  • Oxygen consumption in cultured cells
  • And Many More..

 

EasyRatioPro Applications

PTI--EasyRatioPro Fluorescence Imaging

 

EasyRatioPro

Calcium Measurement

  • Intracellular free Ca2+
  • Mitochondrial Ca2+
  • Endoplasmic reticulum (ER) Ca2+ pool
  • Lysosomal Ca2+
  • Extracellular near membrane Ca2+

Membrane Potential

  • Plasma membrane Potential
  • Mitochondrial Membrane Potential

GFP & Variants

  • Identifying cells coexpressing GFP for analysis
  • Use mitoGFP to visualize mitochondria and measure mitochondrial calcium [Ca2+]m
  • Use ratiometric GFP (redox-GFP) to measure cellular/mitochondria reduce/oxidize reaction
  • HEK-293 cells transfected with the CaSR-pEGFP vector
  • ECFP
  • EYFP

Other Measurements

  • Calcium imaging of Single HEK 293 cell
  • Taste Bud
  • Pea Pollen tubes
  • Respiratory burst & reactive oxygen species (ROS)
  • NAD(P)H
  • Changes in CaM availability
  • Demonstration of selective plasma membrane permeabilization by digitonin in HEK293 cells
  • Detection by immunofluorescence of PfCRT expression in HEK293 cells
  • Receptor-mediated Glutamate release
    (by following NADH signal)
  • Fura-dextra
  • Cellular GSH level
  • FL-aldosterone efflux
  • Latency of response to exogenous ATP or ADP
  • Membrane traffic
  • Liposome
  • ER membrane
  • Cell contractile activity & cell Size
  • Cell Volume Measurement
  • Phagocytosis
  • Cell injury or death by PI fluorescent
  • Metal induced cytotoxity

pH

  • Intracellular pH
  • Vacuolar pH
  • Lysosomal pH

Other Ions

  • Intracellular Mg2+
  • Intracellular Mn2+
  • Intracellular Zn2+
  • Intracellular Sr2+
  • Intracellular Na+
  • Intracellular Cl-
  • Cytosolic labile iron pool (LIP)
  • Iron homeostasis

FRET

  • Donor Excitation Spectroscopy
  • Emission intensity
  • Donor emission intensity
  • Acceptor emission intensity
  • Ratio of donor/acceptor emission intensity
  • Measure molecular proximity
  • Binding of enzyme to its substrate
  • Measure Endoplasmic reticulum (ER) Ca2+

Calcium-related Measurement

  • Ca2+ Waves
  • Ca2+ wave quantitation
  • Ca2+ wave induced bymechanical stimulation
  • Ca2+ wave induced by mechanical wounding
  • Ca2+ wave propagation
  • In non-contacting cells
  • Over a physical gap
  • Ca2+ Oscillation induced by:
    • Hg2+
    • K+
    • Heat
    • ATP
    • Dopamine
    • FSH
    • Caffeine
    • Ca2+ mobilization
    • Ca2+ homeostasis
    • Propagated cytoplasmic Ca2+cyt responses along the DVR wall.
  • Simultaneous Measurement of intracellular Ca2+ and:
    • Cell volume
    • Phygocytosis
    • Cl-
    • Oxidase activation
    • Ca2+-CaM

Non Ratiometric

Application

Dye

Chloride SPQ SPA
MQAE

 

 
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